@article{oai:kyukan.repo.nii.ac.jp:00000368,
 author = {Kumamoto, Toshihide and Nakamura, Kenichiro and Nakano, Yoshikazu and Kawamura, Yuko and Aso, Yasuhiro and Hazama, Yusuke and Kimura, Noriyuki},
 issue = {1},
 journal = {九州看護福祉大学紀要, The journal of Kyushu University of Nursing and Social Welfare},
 month = {Mar},
 note = {110009889137, Objective: We investigated the effect of insulin-like growth factor-1 (IGF-1) on the histological (e.g., rimmed vacuoles and muscle fiber atrophy) and molecular properties of the denervated muscles of chloroquine-treated rats. Methods: The left hind-legs of adult male Wistar rats were denervated by ligation of the sciatic nerve.The rats with and without chloroquine treatment were given saline or IGF-1 intraperitoneally at a daily dose of either 2 or 8 mg/kg for 8 days. Results: In the denervated soleus muscles from chloroquine-treated rats, the number of rimmed vacuoles was significantly reduced in response to either low- or high-dose IGF-1. IGF-1 administration did not affect the muscle fiber atrophy. Although proteasome and ubiquitin immunostaining, and the mRNA levels of muscle-specific ubiquitin ligases and autophagy-related genes were significantly increased in denervated muscles from both rats, those levels did not change in response to either low or high-doses of IGF-1. Conclusion: IGF-1 suppressed rimmemd vacuolar formation in muscle fibers in an animal model of chloroquine-induced myopathy., 目的：脱神経処理したクロロキン投与ラット筋の病理所見および筋崩壊に関与する分子に対するIGF-1の影響を検討した。 
 　方法：予め左側の坐骨神経を結紮（脱神経）したラットにクロロキン、または生理的食塩水（生食水）を各々投与した（各20匹）。そのうち両ラットの各10匹に連日IGF（2 mg/kg、8 mg/kg、各5匹）を投与し、残りの各10匹にIGF-1の代わりに同量の生食水を8日間、腹腔内に注射した。 
 　結果：クロロキン投与ラットの脱神経したヒラメ筋でのみ多数の縁取り空胞（rimmed vacuole）を認め、少量および大量のIGF-1投与によりrimmed vacuoleを有する筋線維数は有意に減少した。IGF-1は筋線維の萎縮には効果はなかった。プロテアソームおよびユビキチンの免疫染色、筋特異的ユビキチンリガーゼおよびオートファジー関連遺伝子のmRNAレベルはクロロキン投与および非投与（生食水投与）の脱神経筋で有意に増加したが、少量および大量のIGF-1投与ではいずれも有意な変化はなかった。 
 　結論：IGF-1は、クロロキン誘発ミオパチーの動物モデルの筋萎縮は抑制しないが、筋線維内のrimmed vacuoleの形成を抑制する。},
 pages = {3--11},
 title = {Administration of insulin-like growth factor-1 inhibits the overdevelopment of chloroquine-induced vacuoles in muscle fibers in experimental rimmed vacuolar myopathy},
 volume = {15},
 year = {2015}
}